Part:BBa_K4831001:Design

MerA (mercuric reductase) from Pseudomonas aeruginosa (seq. harmonized to Synechocystis)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1198
  Illegal NgoMIV site found at 1246
  Illegal AgeI site found at 1484
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The original sequence is harmonized for optimal translation in Synechocystis sp PCC 6803. (changed codons: 406, GC-change: 65.8 to 56.0%).

NOTE! The starting codon ATG has been removed to be compatible with our composite part (reading frame shift).

Source

Part is from Pseudomonas aeruginosa genomic sequence.